Mario Bossi

1989-1993: fellowship granted by Bruno Ceccarelli Center, Department of Pharmacology, Consiglio Nazionale delle Ricerche

Technical skills:

• Thin (microtomy) and ultrathin sectioning (ultramicrotomy) for optical and electronic microscopy

• Electronic and optical micrography

• Epoxy and acrylic resin embedding

• Quick freezing with electrical stimulation

• Cryosubstitution

• Freeze etching in high vacuum systems

• Medium-free fixation methods for Electron Spectroscopic Imaging (E.S.I.)

• Ultracryomicrotomy and immuno electron microscopy

• Immunofluorescence for optical microscopy

1993-2000: electron microscopy technician at  DIBIT, S. Raffaele scientific institute

Technical skills:

• Confocal Microscopy (Zeiss Lsm 01, Biorad mrc 600, Biorad 1024)

• Digital image systems and analysis for  optical microscopy ( software: Lasersharp 2000, Image Pro Plus, Labwiev, Metamorph)

• Metal sputtering and rotary shadowing

• Peroxydase and immunoperoxydase

• Ultrathin serial sectioning and 3d reconstruction

2001-2017: electron microscopy technician at the Department of  Neuroscienze e Tecnologie Biomediche (Now School of Medicine and Surgery)

Technical skills:

• Digital image systems and analysis for  electronic microscopy  ( software: ITEM)

• Digital image systems and analysis for morphometry  (software: Leica Qwin)

• Automatic resin embedding (leica EmTp)

• Imaging deconvolution

• 3d reconstruction in optical microscopy (Biorad Radiance 2100 microscope)

• Negative staining

• Metal sputtering for scanning microscopy

• Elemental Analysis

• Image j software platform – drivers & devices, macros, morphometry

LATEST PUBLICATIONS

• Canta A, Chiorazzi A, Carozzi VA, Meregalli C, Oggioni N, Bossi M, Rodriguez-Menendez V, Avezza F, Crippa L, Lombardi R, de Vito G, Piazza V, Cavaletti G, Marmiroli P.Age-related changes in the function and structure of the peripheral sensory pathway in mice. Neurobiol Aging. 2016 Sep;45:136-148. doi: 10.1016/j.neurobiolaging.2016.05.014. Epub 2016 May 20.

• Ravasi M, Scuteri A, Pasini S, Bossi M, Menendez VR, Maggioni D, Tredici G. Undifferentiated MSCs are able to myelinate DRG neuron processes through p75. Exp Cell Res. 2013 Nov 15;319(19):2989-99. doi: 10.1016/j.yexcr.2013.08.016. Epub 2013 Aug 22.

• Gilardini A, Avila RL, Oggioni N, Rodriguez-Menendez V, Bossi M, Canta A, Cavaletti G, Kirschner DA.Myelin structure is unaltered in chemotherapy-induced peripheral neuropathy. Neurotoxicology. 2012 Jan;33(1):1-7. doi: 10.1016/j.neuro.2011.10.010. Epub 2011 Nov 4.

• Rota Nodari L, Ferrari D, Giani F, Bossi M, Rodriguez-Menendez V, Tredici G, Delia D, Vescovi AL, De Filippis L. Long-term survival of human neural stem cells in the ischemic rat brain upon transient immunosuppression. PLoS One. 2010 Nov 19;5(11):e14035. doi: 10.1371/journal.pone.0014035.

• Bentivegna A, Conconi D, Panzeri E, Sala E, Bovo G, Viganò P, Brunelli S, Bossi M, Tredici G, Strada G, Dalprà L. Biological heterogeneity of putative bladder cancer stem-like cell populations from human bladder transitional cell carcinoma samples. Cancer Sci. 2010 Feb;101(2):416-24. doi: 10.1111/j.1349-7006.2009.01414.x. Epub 2009 Oct 27.

Find out more on

• SCOPUS

• BOA (Bicocca Open Archive)