TECHNICAL EXPERT

Mario Bossi

mario-bossi

Electron microscopy technician at the Department of  Neuroscienze e Tecnologie Biomediche (Now School of Medicine and Surgery)

University of Milano – Bicocca
U8 Building – 1st floor – Room 1034
Via Cadore, 48
20900 – Monza (MB)
Italy

BIOGRAFIA

1989-1993: fellowship granted by Bruno Ceccarelli Center, Department of Pharmacology, Consiglio Nazionale delle Ricerche

Technical skills:

  • Thin (microtomy) and ultrathin sectioning (ultramicrotomy) for optical and electronic microscopy

  • Electronic and optical micrography

  • Epoxy and acrylic resin embedding

  • Quick freezing with electrical stimulation

  • Cryosubstitution

  • Freeze etching in high vacuum systems

  • Medium-free fixation methods for Electron Spectroscopic Imaging (E.S.I.)

  • Ultracryomicrotomy and immuno electron microscopy

  • Immunofluorescence for optical microscopy

1993-2000: electron microscopy technician at  DIBIT, S. Raffaele scientific institute

Technical skills:

  • Confocal Microscopy (Zeiss Lsm 01, Biorad mrc 600, Biorad 1024)

  • Digital image systems and analysis for  optical microscopy ( software: Lasersharp 2000, Image Pro Plus, Labwiev, Metamorph)

  • Metal sputtering and rotary shadowing

  • Peroxydase and immunoperoxydase

  • Ultrathin serial sectioning and 3d reconstruction

2001-2017: electron microscopy technician at the Department of  Neuroscienze e Tecnologie Biomediche (Now School of Medicine and Surgery)

Technical skills:

  • Digital image systems and analysis for  electronic microscopy  ( software: ITEM)

  • Digital image systems and analysis for morphometry  (software: Leica Qwin)

  • Automatic resin embedding (leica EmTp)

  • Imaging deconvolution

  • 3d reconstruction in optical microscopy (Biorad Radiance 2100 microscope)

  • Negative staining

  • Metal sputtering for scanning microscopy

  • Elemental Analysis

  • Image j software platform – drivers & devices, macros, morphometry

 

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